Preparation of Antigen-Specific Tregs for Autoimmune Diseases Therapy

As an important group of suppressive cells in vivo, regulatory T cells (Tregs) play an important role in maintaining immune homeostasis and bring promise for effective therapy of autoimmune diseases. With industry-leading expertise and state-of-the-art equipment, Creative Biolabs provides accurate and effective solutions in terms of the preparation of antigen-specific Tregs for autoimmune disease therapy in the scientific research field.

Regulatory T Cells (Tregs) and Autoimmune Diseases

Tregs are a subset of adaptive lymphocytes. The importance of Tregs in immune regulation and brokering tolerance has been robustly demonstrated. Strategies to induce or increase Tregs have been developed to reduce immune inflammation in patients with autoimmune diseases. Adoptive Treg therapy to treat animal models of autoimmunity has been established, such as experimental autoimmune encephalomyelitis (EAE), arthritis, inflammatory bowel disease.

Fig.1 The mechanisms of action of Tregs. (Jhe, 2018)

How to Prepare Antigen-Specific Tregs?

Tregs play a critical role in regulating the immune system by inhibiting the function of immune cells to keep immunologic self-tolerance and immune homeostasis. An acquired immunodeficiency disease (AID) will occur when the specific transcription factor forkhead box protein p3 (Foxp3) of Tregs is mutated or the CD4+CD25+ T cells are eliminated.

Antigen-specific Foxp3 Tregs can be induced by either expressing antigens in a certain tissue, such as thymic epithelial cells, or delivering antigens under sub-immunogenic conditions. In T cell antigen receptor (TCR)-transgenic mice, scientists implanted osmotic minipumps and found that when supplied 2-week, it could be used to instruct naive T cells to become Foxp3 Tregs.

Fig.2 Potential future applications of Treg cells to target specific tissues and diseases. (Jhe, 2018)

Custom Services of Antigen-Specific Tregs Preparation

Specialized to control auto-immune responses, Tregs are vital in maintaining immune homeostasis. Treg deficits are usually associated with the development of autoimmune diseases. Thus, the expansion of antigen-specific Tregs is viewed as a key step in basic research and clinical application, which can accelerate the research progress of autoimmune diseases. We provide the preparation services of antigen-specific Tregs to advance the therapeutic development of autoimmune diseases. Our comprehensive Treg preparation services include but not limited to:

To study Treg cells, it is necessary to induce them in vitro and make them express in large quantities. Creative Biolabs has many years of experience in the antigen-specific Tregs development process. Our laboratory is equipped with the very latest instrumentation, so we can guarantee the highest quality, accuracy, and precision, and a fast turnaround to meet deadlines. If you have any questions about the preparation of antigen-specific Tregs for autoimmune disease therapy, you can contact us.

Reference

1. Jhe, A.; et al. Regulatory T-cell therapy for autoimmune and autoinflammatory diseases: The next frontier. Journal of Allergy and Clinical Immunology. 2018, 142(6): 1710-1718.

For Research Use Only | Not For Clinical Use